The Bright-Line Hemacytometer is molded from a single piece of thermal and shock-resistant glass. An H-shaped moat forms two counting areas, or plateaus. A "V" slash at the loading side of each plateau facilitates charing and reduces the possibility of overflow into the moat. Each plateau features enhanced Neubauer rulings. The ruled surface is 0.1mm below the cover glass, limiting the volume of blood or fluid over a square mm at 0.1cu.mm and over each of 400 squares (within the central square mm) to 0.00025 cu. mm. Contact of the flat, polished cover glass surfaces with cover glass supports produces an exact volume of fluid over the counting area. The difference in surface tension characteristics between the metallic surface on the chamber and the polished cover glass assures smooth capillarity for precise loading and more even cell distribution.
| Catalog # | Description |
| 1490 | Counting chamber only |
| 1492 | Counting chamber with 2 cover glass |
| 1483 | Complete set |
| 1475 | Phase Counting Chamber w/2 cover glass (used with Phase microscope) |
| 1461 | Replacement Cover Glass |
Directions for Use
Bright-Line / Dark-Line Counting Chambers Catalog Numbers: 3100, 3110, 3200, 3500, 1490, 1492, 1475, & 1483
Usage: Cell Counts
Cell Depth: 0.100mm +/- 2% (1/10mm)
Volume: 0.1 Microliter
Ruling Pattern: Improved Neubauer, 1/400 Square mm
Rulings cover 9 square millimeters. Boundary lines of the Neubauer ruling are the center lines of the groups of three. (These are indicated in the illustration below.) The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. The ruled surface is 0.10mm below the cover glass, so that the volume over each of the 16 small squares is .00025 cubic mm.
The number of cells per cubic millimeter =
Number of cells counted per square millimeter X dilution (if used) X 10
The number of cells per milliliter =
Number of cells counted per square millimeter X dilution (if used) X 10,000
One (1) Milliliter = 1000 cubic millimeters (cu mm)
One (1) Microliter (ul) = One (1) cubic millimeter (cu mm)
To clean the counting chamber: After completing the count, remove the cover glass and clean the counting chamber with water or a mild cleaning solution (10% solution of bleach). Dry the counting chamber with a soft cloth or wipe, or rinse with acetone.
